The diversity of trees, shrubs, herbs, climbers and birds in traditional agroforestry homegarden system contributes to provision of ecosystem services. Homegarden systems involve deliberate management of multipurpose tree species in intimate association with agricultural crops and invariably livestock. This study characterizes the floral and avifaunal diversity and composition in the homegardens of Jaitapur in Konkan coast of Maharashtra. For the investigation 40 homegarden were selected randomly over a 460 km² area for floral diversity analysis and among them 11 homegarden sites selected for seasonal bird diversity analysis. Quadrate sampling technique and point transect survey method based on distance sampling was used for phyto-sociological and bird diversity analysis, respectively. In total, 206 plant and 76 bird species were recorded in the study area. The homegarden vegetation consists of 88 tree, 48 shrub, 44 herb and 26 climber species. The highest number of plant species belongs to Fabaceae family followed by the Apocynaceae and Cucurbitiaceae. The bird density in homegardens was 39 individual’s ha^-1. The study indicates that homegardens contributes to the provision of ecosystem services, such as food, fodder, timber, firewood, vegetables, medicinal values, fertilize soil, carbon sequestration, control pollution and protect environment. The research findings suggest that homegardens of the region are ecologically and ethno-botanically rich. The wide variety of floral and avian species indicates the high species richness and diversity. This study provides a basis for developing measures for the conservation and management of natural resources. The homegardens and surrounding region is prone to drastic anthropogenic land-use changes. The present study conclude that land clearing, land breaking and nuclear power project installation will affect the biodiversity and carbon balance. Therefore, the study suggests that it should not be started at Jaitapur for future environmental health, safety, public health, security and to avoid future hazards of loss of biodiversity.